|
KMID : 0363220070450040332
|
|
Korean Journal of Dermatology
2007 Volume.45 No. 4 p.332 ~ p.337
|
|
|
Effect of Hypoxia on the Melanogenesis of Murine B16 Melanoma Cells
|
|
Song Young-Gil
Lee Seung-Yong
Kim Han-Uk
Yun Seok-Kweon
Yang Jeong-Yeh
Koo Jeung-Hyun
Yoon Ha-Yong
Park Byung-Hyun
Park Jin-Woo
|
|
|
Abstract
|
|
|
Background: Numerous reports suggest the role of oxygen in melanogenesis. However, little has been reported on the effect of a hypoxic environment on cellular melanogenesis.
Objective: The effect of low oxygen tension on cellular melanogenesis was investigated in B16 murine melanoma cells.
Methods: Using cells cultured under an ambient (21% O2) or hypoxic (5% O2) condition, melanin content and tyrosinase activity were measured spectrophotometrically. The expression of tyrosinase, tyrosinase-related protein (TRP)- 1, and TRP-2 were analyzed by RT-PCR and Western blot.
Results: Culture of cells under hypoxic conditions caused significant inhibition of isobutylmethylxanthine (IBMX)- induced increase of melanin content. No cytotoxicity was observed during the hypoxic culture periods. Decreased melanin content occurred through the decrease of tyrosinase protein and activity (p<0.01). The mRNA levels of tyrosinase and TRP-2 were also decreased by hypoxia, while that of TRP-1 was unchanged. Similar inhibitions of melanin content and tyrosinase activity were observed in the cells stimulated with dibutyryl-cAMP.
Conclusion: IBMX-induced melanogenesis in B16 cells was significantly inhibited under hypoxic culture conditions, suggesting the important role of oxygen tension in cellular melanogenesis.
|
|
|
KEYWORD
|
|
|
cAMP, Hypoxia, Melanogenesis, Tyrosinase
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|